pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 5 pasty yellow 5 pasty yellow 0 - pGLO LB/amp 0 0 0 + pGLO LB/amp 0 0 0 + pGLO LB/amp/ara 0 0 0

2.	What two new traits do your transformed bacteria have?
	They don't seem to have any new traits. I beilive our group didn't put the arabinose in the bacteria, therefore our bacteria didn't grow.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	50uL because the bacteria multiplies and there seemed to be just one huge colony that occupied almost the whole tray. It was a lot of bacteria, even though none of it glowed.
4.	What is the role of arabinose in the plates?
	Arabinose activates the gene that make GFP.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Cell marking and selection is one way that scientists use GFP to see which cells picked up the gene and which ones didn't to be a alternative antibiotic. Develepmental and transgenic uses GFP to track fatal cells and stop them. It can also be used for protien purification in case there's an unusual protien that's causing a disruption in your body.
6.	Give an example of another application of genetic engineering. When scientists use fungi to grow the bacteria used in baby cow's stomachs to digest their mom's millk, that's genetic engineering. The bacterica is then used to make the cheese that is so high in demand.